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Biochem Biophys Res Commun. 1996 Aug 5;225(1):326-32.

Fatty acylation of synaptotagmin in PC12 cells and synaptosomes.

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1
Howard Hughes Medical Institute, New Haven, Connecticut, USA.

Abstract

Synaptotagmin I is localized to synaptic vesicles where it functions in the calcium-triggered release of neurotransmitters. Here we demonstrate that synaptotagmin I covalently incorporated [3H]palmitate after metabolic labelling of PC-12 cells and rat brain synaptosomes. Labeling was localized to a tryptic fragment that contains a cluster of cysteine residues adjacent to the molecule's single transmembrane anchor. Neutral hydroxylamine released the [3H]palmitate from this fragment and increased its electrophoretic mobility, demonstrating that acylation occurs at the membrane-proximal cysteine cluster. In addition, hydroxylamine-induced mobility shifts were also apparent for synaptotagmins II and III, suggesting that posttranslational palmitoylation via thioester bonds may be a general modification of all synaptotagmins.

PMID:
8769138
DOI:
10.1006/bbrc.1996.1174
[Indexed for MEDLINE]
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