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Nucleic Acids Res. 1996 Aug 1;24(15):2877-84.

Recruitment of damaged DNA to the nuclear matrix in hamster cells following ultraviolet irradiation.

Author information

1
Department of Biological Sciences, Stanford University, CA 94305-5020, USA.

Abstract

We examined the relationship between the nuclear matrix and DNA in the dihydrofolate reductase domain following irradiation of Chinese hamster cells with UV light. The fraction of matrix-bound DNA increased in transcribed and non-transcribed regions during a 3 h period after irradiation. However, no increase was observed with excision repair-deficient cells mutant for the ERCC1 gene. The major UV-induced lesion, the cyclobutane pyrimidine dimer, increased in frequency in the matrix-bound DNA 1 h after irradiation, in both transcribed and non-transcribed regions, but decreased subsequently. This phenomenon was also lacking in excision repair-deficient cells. These data demonstrate that recruitment of lesion-containing DNA to the nuclear matrix occurs following UV irradiation and suggest that this recruitment is dependent upon nucleotide excision repair. This is consistent with the concept of a 'repair factory' residing on the nuclear matrix at which excision repair occurs.

PMID:
8760868
PMCID:
PMC146037
DOI:
10.1093/nar/24.15.2877
[Indexed for MEDLINE]
Free PMC Article

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