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Plant Mol Biol. 1996 May;31(2):399-403.

A point mutation in the gene encoding the Rubisco large subunit interferes with holoenzyme assembly.

Author information

1
Plant Molecular Physiology Lab., RITE, Kyoto, Japan.

Abstract

Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco), a key enzyme of photosynthetic CO2 fixation, is composed of 8 large and 8 small subunits. The Rubisco-deficient Nicotiana tabacum mutant Sp25 is able to synthesize the peptides for both subunits but does not contain any active holoenzyme. The phenotype is maternally inherited and thus caused by a mutation in the chloroplast genome, which also encodes the Rubisco large subunit. A comparison of the nucleotide sequences of the large subunit gene of the Sp25 mutant with that of the wild-type tobacco revealed a single nucleotide change in the Sp25 mutant. This resulted in an amino acid substitution at Gly-322, which was replaced by serine.

PMID:
8756604
DOI:
10.1007/bf00021801
[Indexed for MEDLINE]

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