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Biochem Biophys Res Commun. 1996 Aug 14;225(2):621-6.

The natural product noformycin is an inhibitor of inducible-nitric oxide synthase.

Author information

1
Department of Enzymology, Merck Research Laboratories, Rahway, New Jersey 07065, USA.

Abstract

Inducible-Nitric oxide synthase (iNOS, EC 1.14.13.39) catalyzes the formation of nitric oxide (NO) and L-citrulline from L-Arg. NADPH and dioxygen. The natural product, (-)-noformycin was found to be a potent, competitive inhibitor of recombinant human iNOS with respect to L-Arg with a Ki = 1.3 +/- 0.3 microM. The reversible binding of noformycin caused a high spin type I spectral perturbation of the iNOS heme group with a Kd = 1.5 +/- 0.2 microM. These results demonstrate that natural products may be a useful source for inhibitors of NO-biosynthesis.

PMID:
8753809
DOI:
10.1006/bbrc.1996.1220
[Indexed for MEDLINE]

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