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Anal Quant Cytol Histol. 1996 Apr;18(2):137-43.

Relationship between adipose stromal-vascular cells and adipocytes in human adipose tissue.

Author information

1
Greenville Hospital System/Clemson University Biomedical Cooperative Program, Clemson University, South Carolina 29634-1912, USA.

Abstract

OBJECTIVE:

To determine the relative amounts of RNA and DNA from adipocytes and adipose stromal-vascular cells to better assess differential gene expression for estrogen receptor and cytochrome P450 aromatase in the two cell fractions of adipose tissue.

STUDY DESIGN:

Colorimetric and fluorometric assays were used to measure total RNA and DNA, and the relative cell numbers as well as the relative contribution of RNA from adipocytes and stromal-vascular cells were assessed.

RESULTS:

Adipocytes and stromal-vascular cells exist in a ratio of 2/1. RNA/DNA ratios are the same in the two cell fractions.

CONCLUSION:

In human subcutaneous adipose tissue, adipocytes are twice as numerous as stromal-vascular cells. The overall transcriptional activity of the cell types appears similar.

PMID:
8744503
[Indexed for MEDLINE]

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