4-(p-Nitrobenzyl)pyridine as a spray reagent for the thin-layer chromatographic detection of dimethyl tetrachloroterphthalate herbicide

J Environ Sci Health B. 1977;12(2):155-61. doi: 10.1080/03601237709372060.

Abstract

The developed thin-layer chromatogram of DCPA (Dacthal) is sprayed with 4-(p-nitrobenzyl)pyridine chromogenic reagent followed by ammonium carbonate solution. The chromatogram is then heated in an over at 130 degrees C for 20 minutes. When cool, the chromatogram is sprayed with tetraethylenepentamine reagent. DCPA spots appear blue against a white background. The sensitivity of this test is about 1 microgram. The procedure is useful to detect DCPA in water and in soil.

MeSH terms

  • Chromatography, Thin Layer
  • Fresh Water / analysis
  • Herbicides / analysis*
  • Indicators and Reagents
  • Nitrobenzenes
  • Pesticide Residues / analysis
  • Phthalic Acids / analysis*
  • Pyridines*
  • Soil / analysis

Substances

  • Herbicides
  • Indicators and Reagents
  • Nitrobenzenes
  • Pesticide Residues
  • Phthalic Acids
  • Pyridines
  • Soil
  • 4-(4-nitrobenzyl)pyridine
  • dimethyl 2,3,5,6-tetrachloroterephthalate