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Electrophoresis. 1996 Mar;17(3):612-6.

Two-dimensional electrophoretic patterns of acute-phase human serum proteins in the course of bacterial and viral diseases.

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1
Dipartimento di Biologia Molecolare, Università degli Studi di Siena, Italy.

Abstract

Acute-phase serum proteins were analyzed by two-dimensional electrophoresis with isoelectric focusing in 3-10 immobilized pH gradients. Most spots were identified by reference to the plasma map in the SWISS-2DPAGE database. Serum amyloid A protein spots were identified by immunoblotting with specific antiserum and by matching determined with predicted values of electrophoretic parameters. Changes in the concentrations of alpha 1-antitrypsin, leucine-rich glycoprotein, haptoglobin, serum retinol-binding protein and transthyretin were quantitated by densitometry of silver-stained gels. Electrophoretic patterns from 18 patients with bacterial diseases and 16 patients with viral diseases were compared. The incidence of serum amyloid A protein spots was 18/18 in bacterial diseases and 6/16 in viral diseases. As the the other reactants studied, variations were simultaneous in bacterial disease and tended to be staggered in viral diseases.

PMID:
8740187
DOI:
10.1002/elps.1150170333
[Indexed for MEDLINE]

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