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Lipids. 1996 May;31(5):535-9.

An improved method for rapid analysis of the fatty acids of glycerolipids.

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Department of Agricultural Chemistry, Kyoto Prefectural University, Japan.

Erratum in

  • Lipids 1996 Aug;31(8):889.


An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH-catalyzed transesterification and high-speed gas chromatography. Glycerolipids (20-40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature for 1-2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP-2340 at 240 degrees C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed within 5 min for common vegetable oils and 8 min for fish oils.

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