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Br J Vener Dis. 1979 Apr;55(2):105-8.

Double-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test.

Abstract

The fluorescent treponemal antibody absorption (FTA-ABS) double-staining procedure was reproducible, comparable to the conventional test, and easy to read. We recommend the use of the FTA-ABS double-staining procedure for microscopes with incident illumination, the 100 x/1.30 oil achromatic objective and the 6.3 x ocular to obtain optimal fluorescence, and the KP560 as a barrier filter to exclude rhodamine emission when fluorescein fluorescence is read. With this system, errors related to poor focusing or failure to visualise treponemes on all smears should be eliminated.

PMID:
87240
PMCID:
PMC1045600
DOI:
10.1136/sti.55.2.105
[Indexed for MEDLINE]
Free PMC Article

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