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Plant Cell Physiol. 1996 Jan;37(1):103-6.

Trans-kingdom conjugation between Agrobacterium tumefaciens and Saccharomyces cerevisiae, a bacterium and a yeast.

Author information

1
Department of Biological Science, Hiroshima University, Japan. 515582/ue.ipc.hiroshima-u.ac.jp

Abstract

For conjugation between prokaryotic Agrobacterium tumefaciens and eukaryotic Saccharomyces cerevisiae, we constructed two novel conjugative plasmids. A. tumefaciens transmitted the plasmids to S. cerevisiae with the aid of tra genes on a helper plasmid. The transmitted plasmids retained their original structure and function in transconjugant yeasts. The presence of Ti plasmid barely affected the trans-kingdom conjugation.

PMID:
8720926
[Indexed for MEDLINE]

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