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Appl Microbiol Biotechnol. 1996 Jan;44(5):589-96.

Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90.

Author information

1
Fermentation Center, Takeda Chemical Industries Ltd., Yodogawa-ku, Osaka, Japan.

Abstract

Determination of the nucleotide sequence downstream from the Lysobacter lactamgenus pcbC gene encoding isopenicillin N synthase revealed that five open-reading frames (ORF) including the pcbC gene were tightly linked in the same orientation. Each ORF has the remarkable feature of the protein-coding frame in the DNA sequence with a high G+C content. Expression in Escherichia coli and a comparison of the deduced amino acid sequences with published sequences showed that the gene cluster contained a deacetoxycephalosporin C synthetase (DAOCS) gene (cefE), an ORF having homology with the Cephalosporium acremonium DAOCS/deacetylcephalosporin C synthetase gene (cefEF), an isopenicillin N epimerase gene(cefD), and a beta-lactamase gene. The gene order was pcbC-cefE-ORF3-cefD-beta-lactamase.

PMID:
8703429
DOI:
10.1007/bf00172490
[Indexed for MEDLINE]

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