Format

Send to

Choose Destination
See comment in PubMed Commons below
J Biol Chem. 1996 Aug 23;271(34):20726-33.

Nucleosome disruption by human SWI/SNF is maintained in the absence of continued ATP hydrolysis.

Author information

1
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.

Abstract

We have examined the requirement for ATP in human (h) SWI/SNF-mediated alteration of nucleosome structure and facilitation of transcription factor binding to nucleosomal DNA. hSWI/SNF-mediated nucleosome alteration requires hydrolysis of ATP or dATP. The alteration is stable upon removal of ATP from the reaction or upon inhibition of activity by excess ATPgammaS, indicating that continued ATP hydrolysis is not required to maintain the altered nucleosome structure. This stable alteration is sufficient to facilitate binding of a transcriptional activator protein; concurrent ATP hydrolysis was not required to facilitate binding. These data suggest sequential steps that can occur in the process by which transcription factors gain access to nucleosomal DNA.

PMID:
8702824
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Support Center