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J Reprod Fertil. 1996 Mar;106(2):321-7.

Metabolism of glycerol 3-phosphate by mature boar spermatozoa.

Author information

1
Department of Biochemistry, University of Sydney, NSW, Australia.

Abstract

Under anaerobic conditions boar spermatozoa metabolized fructose and glucose to lactate but did not produce ATP to the extent of that produced under aerobic conditions; the ketogenic amino acids leucine, tryptophan, phenylalanine and tyrosine were not oxidatively metabolized. Glycerol 3-phosphate was metabolized rapidly in the presence or absence of the glycolytic inhibitor, 3-chloro-1-hydroxypropanone (CHOP). In the absence of CHOP, glycerol 3-phosphate was converted to CO2, lactate, glucose 6-phosphate and fructose 6-phosphate, and ATP was produced. In the presence of CHOP, glycerol 3-phosphate did not produce CO2, lactate or ATP, but formed fructose 1,6-bisphosphate and dihydroxyacetone phosphate. With dihydroxyacetone phosphate as substrate, fructose 1,6-bisphosphate, lactate, glucose 6-phosphate, fructose 6-phosphate and ATP were produced. Accumulation of glucose 6-phosphate and fructose 6-phosphate from glycerol 3-phosphate appeared to depend on the production of ATP; if ATP was not produced, dihydroxyacetone phosphate and fructose 1,6-bisphosphate accumulated. The conversion of glycerol 3-phosphate to glycolytic intermediates appeared to be a mechanism for the conversion of substrates for the ultimate production of lactate.

PMID:
8699417
DOI:
10.1530/jrf.0.1060321
[Indexed for MEDLINE]

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