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Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6231-5.

Defective STAT signaling by the leptin receptor in diabetic mice.

Author information

1
Department of Pharmacology, Biozentrum, University of Basel, Switzerland.

Abstract

Leptin and its receptor, obese receptor (OB-R), comprise an important signaling system for the regulation of body weight. Splice variants of OB-R mRNA encode proteins that differ in the length of their cytoplasmic domains. We cloned a long isoform of the wild-type leptin receptor that is preferentially expressed in the hypothalamus and show that it can activate signal transducers and activators of transcription (STAT)-3, STAT-5, and STAT-6. A point mutation within the OB-R gene of diabetic (db) mice generates a new splice donor site that dramatically reduces expression of this long isoform in homozygous db/db mice. In contrast, an OB-R protein with a shorter cytoplasmic domain is present in both db/db and wild-type mice. We show that this short isoform is unable to activate the STAT pathway. These data provide further evidence that the mutation in OB-R causes the db/db phenotype and identify three STAT proteins as potential mediators of the anti-obesity effects of leptin.

PMID:
8692797
PMCID:
PMC39004
DOI:
10.1073/pnas.93.13.6231
[Indexed for MEDLINE]
Free PMC Article

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