Send to

Choose Destination
Biochem Biophys Res Commun. 1996 Jun 25;223(3):691-4.

Retinoic acid promotes neural and represses mesodermal gene expression in mouse embryonic stem cells in culture.

Author information

Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.


Mouse embryonic stem cells treated with retinoic acid are induced to differentiate into neuron-like cells (Bain et al. (1995) Dev. Biol. 168, 342-357). Here we have examined the expression of a set of neural- and mesoderm-specific genes during this in vitro differentiation process. mRNAs encoding the neural genes Wnt-1, MASH1, the light and medium isoforms of neurofilaments, and the neurotransmitter-synthesizing enzyme glutamic acid decarboxylase are all strongly upregulated by retinoic acid treatment; expression of these genes occurs in a temporal pattern resembling that in the developing brain. In contrast, retinoic acid blocks the expression of the mesodermal genes Brachyury, cardiac actin, and zeta-globin. Thus, retinoic acid exerts both pro-neuronal and anti-mesodermal activities on mouse embryonic stem cells in culture.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center