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Nature. 1996 Aug 1;382(6590):420-5.

Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.

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1
Mitotic Spindle Group in the Cell Biology Program, EMBL, Heidelberg, Germany.

Abstract

Functional nuclei and mitotic spindles are shown to assemble around DNA-coated beads incubated in Xenopus egg extracts. Bipolar spindles assemble in the absence of centrosomes and kinetochores, indicating that bipolarity is an intrinsic property of microtubules assembling around chromatin in a mitotic cytoplasm. Microtubules nucleated at dispersed sites with random polarity rearrange into two arrays of uniform polarity. Spindle-pole formation requires cytoplasmic dynein-dependent translocation of microtubules across one another. It is proposed that spindles form in the absence of centrosomes by motor-dependent sorting of microtubules according to their polarity.

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PMID:
8684481
DOI:
10.1038/382420a0
[Indexed for MEDLINE]
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