Abstract
In Escherichia coli, repression of the aceBAK operon is mediated by the IclR protein. We used an in vitro oligonucleotide selection technique to determine the consensus recognition sequence for MR. Mutational analysis confirmed the contribution of this sequence to repression in vivo and identified the -35 element of the promoter.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / metabolism*
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Base Sequence
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Binding Sites
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Consensus Sequence
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DNA, Bacterial / metabolism*
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DNA-Binding Proteins / metabolism*
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins*
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Isocitrate Lyase / genetics
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Malate Synthase / genetics
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Molecular Sequence Data
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Mutagenesis
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Operon*
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Phosphoprotein Phosphatases / genetics
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Promoter Regions, Genetic*
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Protein Serine-Threonine Kinases / genetics
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Repressor Proteins / metabolism*
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Transcription Factors*
Substances
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Bacterial Proteins
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DNA, Bacterial
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DNA-Binding Proteins
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Escherichia coli Proteins
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Repressor Proteins
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Transcription Factors
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IclR protein, E coli
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Malate Synthase
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Protein Serine-Threonine Kinases
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isocitrate dehydrogenase kinase
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isocitrate dehydrogenase phosphatase
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Phosphoprotein Phosphatases
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Isocitrate Lyase