Oxidant-mediated proteolytic activation of Ca(+)-ATPase in microsomes of pulmonary smooth muscle

S K Ghosh; T Chakraborti; J R Michael; S Chakraborti

doi:10.1016/0014-5793(96)00471-1

Oxidant-mediated proteolytic activation of Ca(+)-ATPase in microsomes of pulmonary smooth muscle

FEBS Lett. 1996 Jun 3;387(2-3):171-4. doi: 10.1016/0014-5793(96)00471-1.

Authors

S K Ghosh¹, T Chakraborti, J R Michael, S Chakraborti

Affiliation

¹ Department of Biochemistry and Biophysics, University of Kalyani, India.

PMID: 8674543
DOI: 10.1016/0014-5793(96)00471-1

Abstract

Treatment of bovine pulmonary artery smooth muscle tissue microsomes with H2O2 (1 mM) markedly stimulated protease activity tested with a synthetic substrate N-benzoyl-DL-arginine p-nitroanilide (BAPNA), and also enhanced Ca(2+)-ATPase activity. ATP-dependent Ca(2+) uptake was found to be stimulated upon treatment of the microsomes with H2O2. Pretreatment of the microsomes with vitamin E and aprotinin prevented the H2O2-induced stimulation of Ca(2+)-ATPase activity and also ATP-dependent Ca(2+) uptake. In contrast, H2O2-induced inhibition of Na(+)-dependent Ca(2+) uptake was reversed by vitamin E and aprotinin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

5'-Nucleotidase / metabolism
Acid Phosphatase / metabolism
Animals
Aprotinin / pharmacology
Calcium / metabolism
Calcium-Transporting ATPases / drug effects
Calcium-Transporting ATPases / metabolism*
Cattle
Electron Transport Complex IV / metabolism
Enzyme Activation
Hydrogen Peroxide / pharmacology*
Microsomes / drug effects
Microsomes / enzymology*
Muscle, Smooth, Vascular / enzymology*
NADH, NADPH Oxidoreductases / metabolism
NADPH-Ferrihemoprotein Reductase
Oxidants / pharmacology*
Pulmonary Artery
Vitamin E / pharmacology

Substances

Oxidants
Vitamin E
Aprotinin
Hydrogen Peroxide
NADH, NADPH Oxidoreductases
NADPH-Ferrihemoprotein Reductase
Electron Transport Complex IV
Acid Phosphatase
5'-Nucleotidase
Calcium-Transporting ATPases
Calcium