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Neuron. 1996 Jun;16(6):1189-96.

Cloning and functional expression of a human Ca2+-permeable cation channel activated by calcium store depletion.

Author information

1
Institut für Pharmakologie, Freie Universität Berlin, Federal Republic of Germany.

Abstract

Depletion of intracellular calcium stores generates a signal that activates Ca2+-permeable channels in the plasma membrane. We have identified a human cDNA, TRPC1A, from a human fetal brain cDNA library. TRPC1A is homologous to the cation channels trp and trpl in Drosophila and is a splice variant of the recently identified cDNA Htrp-1. Expression of TRPC1A in CHO cells induced nonselective cation currents with similar permeabilities for Na+, Ca2+, and Cs+. The currents were activated by intracellular infusion of myo inositol 1,4,5-trisphosphate or thapsigargin. Expression of TRPC1A significantly enhanced increases in the intracellular free calcium concentration induced by Ca2+ restitution after prolonged depletion. Similar results were obtained in Sf9 cells. We conclude that TRPC1A encodes a Ca2+-permeable cation channel activated by depletion of intracellular calcium stores.

PMID:
8663995
DOI:
10.1016/s0896-6273(00)80145-2
[Indexed for MEDLINE]
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