Format

Send to

Choose Destination
See comment in PubMed Commons below
Neuron. 1996 Jun;16(6):1159-67.

Voltage-sensing residues in the S2 and S4 segments of the Shaker K+ channel.

Author information

1
Department of Physiology, School of Medicine, University of California, Los Angeles 90095-1751, USA.

Abstract

The activation of Shaker K+ channels is steeply voltage dependent. To determine whether conserved charged amino acids in putative transmembrane segments S2, S3, and S4 contribute to the gating charge of the channel, the total gating charge movement per channel was measured in channels containing neutralization mutations. Of eight residues tested, four contributed significantly to the gating charge: E293, an acidic residue in S2, and R365, R368, and R371, three basic residues in the S4 segment. The results indicate that these residues are a major component of the voltage sensor. Furthermore, the S4 segment is not solely responsible for gating charge movement in Shaker K+ channels.

PMID:
8663992
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center