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Biochem Biophys Res Commun. 1996 Jun 5;223(1):64-72.

An upstream positive regulatory element in human GM-CSF promoter is recognized by NF-kappa B/Rel family members.

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IBiG, Institute of Biology and Genetics, University of Genova, Italy.


To further extend the previous analysis of cis-acting elements and cognate trans-acting factors that contribute to GM-CSF transcriptional regulations, we have examined a promoter region between -1742 and -2010. DNase I footprinting assays showed four protected sequences named A, B, C and D. DNA transfections in the T-lymphoid Mo cell line, which constitutively expresses GM-CSF, indicated that the A element, located between -2002 and -1984, has a positive role on transcription. Further characterization by electrophoretic mobility shift assays in the presence of different competitor oligonucleotides showed that this element binds a factor of the NF-kappa B/Rel family.

[Indexed for MEDLINE]

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