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Mutat Res. 1996 Feb 19;350(1):217-27.

Analysis of the mammalian recombination protein complex RC-1.

Author information

1
Basel Institute for Immunology, Switzerland.

Abstract

Based on a novel cell-free assay for DNA recombination, we previously reported the purification and initial characterization of RC-1, a protein complex catalyzing the recombinational repair of deletions and gaps. RC-1 was isolated from calf thymus nuclear extracts and shown to copurify with several enzymatic activities, among them a DNA polymerase. Here, additional evidence is reported identifying the polymerase as DNA polymerase epsilon. Furthermore, a novel DNA structure-dependent endonuclease associated with RC-1 was observed, which recognizes and cleaves branched DNA substrates at specific sites. Implications of this endonuclease activity for the recombination reaction are discussed.

PMID:
8657184
DOI:
10.1016/0027-5107(95)00106-9
[Indexed for MEDLINE]

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