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Gene. 1996 Jun 12;172(1):169-70.

Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae.

Author information

1
Departamento de BiologĂ­a Molecular de Plantas, UNAM, Cuernavaca, Morelos, Mexico.

Abstract

We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.

PMID:
8654982
DOI:
10.1016/0378-1119(96)00059-5
[Indexed for MEDLINE]

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