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Mol Gen Genet. 1977 Feb 28;151(1):77-81.

Protoplast fusion of Schizosaccharomyces pombe Auxotrophic mutants of identical mating-type.

Abstract

Protoplasts of methionine- and lysine-requiring h- mutants isolated from the L972 h- strain of Schizosaccharomyces pombe were fused. The protoplasts were obtained from the cells with enzymes produced by Trichoderma viride. When a mixture of the protoplasts was treated with 30% PEG 4000 solution containing 10 mM CaCl2, cell fusion and complementation was attained with a frequency of 0.17%. Both fusion partners were recovered among the spores after crossing of the fusion products with the strain M210 ade6 h+. Cytological and haploidization examinations showed that the fusion cells are not heterokaryons, and that the increased amount of genetic material is situated in one nucleus.

PMID:
865481
DOI:
10.1007/bf00446915
[Indexed for MEDLINE]

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