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FEBS Lett. 1996 May 20;386(2-3):141-8.

Flow cytometry analysis of alpha1-adrenoceptor subtypes.

Author information

1
Department of Molecular, Cell Pharmacology, National Children's Medical Research Center, Tokyo, Japan.

Abstract

To characterize the alpha1-adrenoceptor subtypes, we developed a flow cytometry method using the fluorescent ligand BODIPY-FL prazosin and the anti-peptide antibody against the alpha1b-adrenoceptor amino terminus (designated 1B-N1-C) as probes. Three alpha1-adrenoceptors (alpha1a, alpha1b and alpha1d) expressed in CHO cells were detected by BODIPY-FL prazosin; however, only alpha1b-adrenoceptor subtype was detected by the anti-peptide antibody 1B-N1-C. Furthermore, the flow cytometry analysis with 1B-N1-C specifically identified alpha1b-adrenoceptor in native cells of hamster DDT1-MF2 cells, rat hepatocytes and cardiomyocytes.

PMID:
8647269
[Indexed for MEDLINE]
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