Format

Send to

Choose Destination
Nat Genet. 1996 Jun;13(2):167-74.

Identification and mapping of human cDNAs homologous to Drosophila mutant genes through EST database searching.

Author information

1
Telethon Institute of Genetics and Medicine (TIGEM) Milan, Italy.

Abstract

Cross-species comparison is an effective tool used to identify genes and study their function in both normal and pathological conditions. We have applied the power of Drosophila genetics to the vast resource of human cDNAs represented in the expressed sequence tag (EST) database (dbEST) to identify novel human genes of high biological interest. Sixty-six human cDNAs showing significant homology to genes causing Drosophila mutant phenotypes were identified by screening dbEST using the "text string' option, and their map position was determined using both fluorescence in situ hybridization (FISH) and radiation hybrid mapping. Comparison between these genes and their putative partners in Drosophila may provide important insights into their function in mammals. Furthermore, integration of these genes into the transcription map of the human genome contributes to the positional candidate approach for disease gene identification.

Comment in

PMID:
8640222
DOI:
10.1038/ng0696-167
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center