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Arthritis Rheum. 1996 May;39(5):736-45.

Detection of Borrelia burgdorferi by DNA amplification in synovial tissue samples from patients with Lyme arthritis.

Author information

1
Institut de Bactériologie, Université Louis Pasteur, Strasbourg, France.

Abstract

OBJECTIVE:

To compare the detection rates of chromosomal flagellin gene from Borrelia burgdorferi in synovial tissue (ST) and synovial fluid (SF) using polymerase chain reaction (PCR) techniques.

METHODS:

B burgdorferi DNA was sought in SF and ST from 12 consecutive patients with Lyme arthritis and from 29 patients with noninfectious diseases (controls).

RESULTS:

No DNA amplification was observed in samples obtained from the 29 control patients, whereas B burgdorferi DNA was detected in all ST and/or SF samples from the 12 patients with Lyme arthritis. Results from 1 ST sample were not interpretable because of PCR inhibitors. Among the 11 remaining patients, 10 had positive ST samples, whereas only 4 had positive SF samples (P < 0.05).

CONCLUSION:

These data suggest that detection of chromosomal B burgdorferi DNA may be more efficient in ST than SF.

PMID:
8639170
DOI:
10.1002/art.1780390505
[Indexed for MEDLINE]

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