Abstract
Replication fork pause (RFP) sites transiently arresting replication fork movement were mapped to transfer RNA (tRNA) genes of Saccharomyces cerevisiae in vivo. RFP sites are polar, stalling replication forks only when they oppose the direction of tRNA transcription. Mutant tRNA genes defective in assembly of transcription initiation complexes and a temperature-sensitive RNA polymerase III mutant (rpc160-41) defective in initiation of transcription do not stall replication forks, suggesting that transcription is required for RFP activity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Cloning, Molecular
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DNA Replication*
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Genes, Fungal
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Molecular Sequence Data
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Point Mutation
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RNA Polymerase III / metabolism
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RNA, Fungal / genetics*
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RNA, Transfer / genetics*
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Repetitive Sequences, Nucleic Acid
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Replication Origin
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Sequence Deletion
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Temperature
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Transcription Factors / metabolism
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Transcription, Genetic*
Substances
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RNA, Fungal
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Transcription Factors
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RNA, Transfer
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RNA Polymerase III