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Am J Vet Res. 1996 Feb;57(2):163-7.

Antigenic and restriction enzyme analysis of Campylobacter spp associated with abortion in sheep.

Author information

1
Caine Veterinary Teaching and Research Center, Department of Animal and Veterinary Science, University of Idaho, Caldwell 83605, USA.

Abstract

OBJECTIVE:

To determine the scope of strain variations among Campylobacter spp associated with abortion in sheep.

DESIGN:

To examine Campylobacter spp isolated from cases of abortion for biochemical, antigenic, and genetic differences.

SAMPLE POPULATION:

15 isolates of Campylobacter spp isolated from cases of abortion during a single lambing season.

PROCEDURE:

Isolates were examined, using biochemical tests, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane proteins (OMP), and DNA restriction enzyme analysis (REA).

RESULTS:

Eight strain variants were detected among the 15 isolates. 14 of the isolates were C jejuni, 13 of which were biotype I and 1 biotype II, and the remaining isolate was identified as C fetus subsp fetus. Five sodium dodecyl sulfate-polyacrylamide gel electrophoresis OMP patterns had distinctive profiles for C fetus subsp fetus and C jejuni biogroup-II isolates and 3 variants within the C jejuni biogroup-I isolates. Examination of REA patterns of DNA from the 15 isolates digested with Cfo I indicated clear differences correlating with species and biogroups and 4 REA variants among biotype-I isolates.

CONCLUSIONS:

Marked antigenic and genetic heterogeneity of Campylobacter isolates were associated with ovine abortion within a defined geographic area.

CLINICAL RELEVANCE:

Representatives of differing OMP and REA profile groups should be considered for incorporation in vaccines to optimize protection in this region and possibly other geographic areas.

PMID:
8633801
[Indexed for MEDLINE]

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