Abstract
This study examined the variety of obligate and facultative anaerobic bacterial species recovered from cases of acute apical periodontitis. A total of 19 root canal samples and 24 periapical granuloma samples were taken from patients suffering pain and discomfort. Bacteria were identified by applying the following techniques: culturing on various media, Gram-staining and using commercially available biochemical test strips. In addition, Prevotella intermedia and Porphyromonas endodontalis were differentiated on a molecular genetic level using species-specific oligodeoxynucleotide probes. The most frequently identified bacteria were Prevotella intermedia, Bifidobacterium spp., Streptococcus sanguis, Streptococcus milleri-group and Bacteroides spp. Obligate anaerobes occurred at a rate of 82.3%, and the average number of isolates was 6.4 per sample.
MeSH terms
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Actinomyces / isolation & purification
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Adolescent
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Adult
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Bacterial Typing Techniques
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Bacteroides / isolation & purification
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Bifidobacterium / isolation & purification
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Capnocytophaga / isolation & purification
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Child
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DNA Probes
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DNA, Bacterial / analysis
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Dental Pulp Cavity / microbiology
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Eubacterium / isolation & purification
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Female
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Fusobacterium / isolation & purification
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Gram-Negative Anaerobic Bacteria / isolation & purification
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Humans
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Male
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Middle Aged
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Periapical Granuloma / microbiology*
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Periapical Periodontitis / microbiology
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Porphyromonas / isolation & purification
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Prevotella / isolation & purification
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Propionibacterium / isolation & purification
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Pulpitis / microbiology*
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Staphylococcus / isolation & purification
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Streptococcus / isolation & purification
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Veillonella / isolation & purification
Substances
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DNA Probes
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DNA, Bacterial