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Am J Ophthalmol. 1996 Feb;121(2):221-3.

Postmortem diagnosis of Fusarium panophthalmitis by the polymerase chain reaction.

Author information

1
Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, CT 06520, USA.

Abstract

PURPOSE:

We undertook this study to determine if a polymerase chain reaction-based test that we developed for the filamentary fungus, Fusarium, could be used to detect the organism in postmortem ocular tissues.

METHODS:

We applied the polymerase chain reaction to amplify a target fragment of Fusarium DNA from formalin-fixed ocular tissues from a patient with endogenous Fusarium panophthalmitis.

RESULTS:

By using the polymerase chain reaction-based test, we were able to amplify the target fragment of DNA from the infected eyes, but not from uninfected control eyes.

CONCLUSIONS:

The technique appears to hold promise to be a sensitive, specific, and rapid method of diagnosing Fusarium infections.

PMID:
8623899
DOI:
10.1016/s0002-9394(14)70594-x
[Indexed for MEDLINE]

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