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Gene. 1996 Apr 17;170(1):147-8.

Construction of an alkaline phosphatase fusion-generating transposon, mTn10phoA.

Author information

1
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620, USA. mcclain@umich.edu.

Abstract

We constructed a derivative of the mini-transposon mTn10 that generates translational fusions to the phoA gene from Escherichia coli and carries the KmR determinant from Tn5. This new transposon, mTn10phoA, is carried on a mobilizable plasmid with both selectable and counterselectable markers. The plasmid carrying mTn10phoA was introduced into Legionella pneumophila. Southern hybridization analysis indicated that the mTn10phoA insertions were randomly distributed.

PMID:
8621078
DOI:
10.1016/0378-1119(95)00856-x
[Indexed for MEDLINE]

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