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FEBS Lett. 1996 Mar 11;382(1-2):203-8.

The micelle to vesicle transition of lipids and detergents in the presence of a membrane protein: towards a rationale for 2D crystallization.

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1
Institute of Cell Biology, ETH, Z├╝rich, Switzerland.

Abstract

The assembly of two-dimensional membrane protein crystals in the presence of lipids was analyzed with quasielastic light scattering and electron microscopy. Mixtures of detergent-solubilized lipids and/or proteins were submitted to slow or rapid dilution while measuring the hydrodynamic radii of the aggregates. Lipids alone exhibited lambda-shaped dilution curves with intermediate rod-shaped particles that converted into small vesicles. Depending on the protein-protein and protein-lipid interactions, detergent-solubilized protein-lipid mixtures showed a sharp transition from micelles to large densely packed proteoliposomes. Electron microscopy revealed that formation of crystals occurred shortly after this phase transition.

PMID:
8612753
DOI:
10.1016/0014-5793(96)00180-9
[Indexed for MEDLINE]
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