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J Chromatogr B Biomed Appl. 1995 Nov 17;673(2):159-64.

Determination of epitestosterone and testosterone in urine by high-performance liquid chromatography.

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Laboratorio de Control de Dopaje, Consejo Superior de Deportes, Madrid, Spain.


This paper describes an alternative HPLC method for the determination of testosterone and epitestosterone, which is simple, rapid, selective, sensitive and reproducible. Samples were prepared using a previous enzymatic hydrolysis with liquid-liquid extraction. The determination was carried out on a Hypersil BDS-C18 reversed-phase column with gradient elution and UV absorbance detection (240 nm). The limits of quantification (signal-to-noise ratio = 6) were 20 ng/ml for testosterone and 30 ng/ml for epitestosterone.

[Indexed for MEDLINE]

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