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Vet Microbiol. 1995 Nov;47(1-2):191-7.

Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils.

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Danish Veterinary Laboratory, Copenhagen V, Denmark.


In order to isolate Actinobacillus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropneumoniae. Following optimization of the media 96 isolates of A. pleuropneumoniae were tested on S-TSA and S-MBA. For isolation of A. pleuropneumoniae from 101 pig tonsils collected at slaughter the S-MBA proved significantly better than both S-TSA and the two non-selective agars tested. Furthermore, contaminating to S-TSA and the two non-selective media.

[Indexed for MEDLINE]

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