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Arch Pediatr Adolesc Med. 1996 Mar;150(3):245-8.

Optical immunoassay for rapid detection of group A beta-hemolytic streptococci. Should culture be replaced?

Author information

1
Department of Pediatrics, University of Virginia Medical Center, Charlottesville, USA.

Abstract

OBJECTIVE:

To evaluate an optical immunoassay rapid antigen test to detect group A beta-hemolytic streptococci directly from throat swab specimens.

DESIGN:

Criterion standard with "blinded" comparison. Double-swab pharyngeal samples were obtained; one swab was cultured and the other was used for the rapid antigen test.

SETTING:

Microbiology laboratory in a primary care center at a university teaching hospital.

PATIENTS:

Two hundred sixty-two outpatients with pharyngitis.

MAIN OUTCOME MEASURE:

The results of the optical immunoassay rapid antigen test were compared with results of standard aerobic culture.

RESULTS:

Sixty-two (24%) of 262 samples were culture-positive; 48 of these 62 were positive by the optical immunoassay rapid test (sensitivity, 77%). Of the 200 culture-negative samples, seven (4%) were positive by the rapid test (specificity, 96%).

CONCLUSION:

The optical immunoassay performed well, but like other rapid tests, is not sensitive enough to replace standard culture for detection of group A beta-hemolytic streptococci.

PMID:
8603215
[Indexed for MEDLINE]

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