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Virology. 1996 Mar 1;217(1):211-9.

HIV-1 infection of cultured human adult oligodendrocytes.

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1
Graduate Group in Molecular Biology, Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia 19104-6146, USA.

Abstract

The mechanism through which HIV-1 causes HIV dementia (HIVD) is not well understood. Myelin pallor is a common pathological finding in HIVD and could be explained by a direct infection of oligodendrocytes or interaction with HIV-1 gp 120. To determine if oligodendrocytes could be infected by HIV-1, we purified oligodendrocytes from adult human brain tissues obtained from temporal lobe resections. These cells were exposed to HIV-1 and infectivity was assayed by detection of p24gag antigen, PCR amplification, and cocultivation with CD4+ cells. These results indicate that HIV-1(IIIB and BaL) and one of four primary isolates tested can infect oligodendrocytes, resulting in the production of infectious virus. Furthermore, in an experiment that mimics a potential in vivo scenario, infected microglia were able to transmit virus to oligodendrocytes in a trans-well culture system. These experiments indicate that oligodendrocyte infection should be considered in studying the pathophysiology of HIVD.

PMID:
8599205
DOI:
10.1006/viro.1996.0108
[Indexed for MEDLINE]
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