Format

Send to

Choose Destination
Virology. 1996 Mar 1;217(1):147-57.

Interaction between cauliflower mosaic virus inclusion body protein and capsid protein: implications for viral assembly.

Author information

1
Friedrich Miescher Institute, Basel, Switzerland.

Abstract

The cauliflower mosaic virus (CaMV) inclusion body protein (pVI) is able to specifically interact with the viral capsid precursor protein (pIV). By using the yeast two-hybrid system and a blot assay, the pIV region required for the recognition of pVI was mapped to the lysine-rich domain. This region of only 48 amino acids when fused to dihydrofolate reductase (DHFR) mediated pVI and DNA binding in vitro. Competition experiments confirmed that pVI and DNA bind to the same region of pIV. Since pVI is absent from the mature virus, models are discussed in which pVI plays an accessory role in CaMV assembly, in addition to its function in transactivating translation.

PMID:
8599199
DOI:
10.1006/viro.1996.0102
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center