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FEMS Microbiol Lett. 1996 Jan 15;135(2-3):187-9.

Comparison of assays for Cryptosporidium parvum oocysts viability after chemical disinfection.

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Department of Civil Engineering, University of Alberta, Edmonton, Canada.


In vitro excystation, vital dyes (4', 6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI), and infectivity in neonatal CD-1 mice were used to assess the viability of Cryptosporidium parvum oocysts after chemical disinfection. In vitro excystation and DAPI/PI staining provided similar estimates of viability in bench-scale experiments, but both of these methods significantly overestimated the viability when compared with infectivity (Pr < or = 0.01). Infectivity was the most reliable measure of the viability of C. parvum oocysts following chemical disinfection.

[Indexed for MEDLINE]

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