Format

Send to

Choose Destination
J Neurobiol. 1995 Dec;28(4):433-44.

Gap junctions are required for the propagation of spreading depression.

Author information

1
Department of Anatomy and Cell Biology, New York Medical College, Valhalla 10595, USA.

Abstract

Spreading depression (SD) is a slowly propagating depression of cerebral neuronal activity and transmembrane ionic gradients, that arises in response to a variety of noxious stimuli. SD bears a strong resemblance to gap junction-mediated calcium waves among cultured astrocytes. Here, we show that gap junction-mediated intercellular diffusion is necessary for the generation of SD. Waves of SD in the isolated chicken retina were blocked by five different inhibitors of gap junctional coupling, which was assessed by the intercellular transit of Lucifer Yellow (LY). Each of these gap junction blockers inhibited both the migration of SD and the diffusion of LY in a dose-dependent manner. In contrast, glutamate-evoked calcium influx into retinal cells was not affected by these compounds. The results indicate that intercellular coupling through gap junctions is required for SD. Gap junction-mediated communication might therefore constitute an important mechanism in both normative and pathological brain function.

PMID:
8592104
DOI:
10.1002/neu.480280404
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center