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Int J Obes Relat Metab Disord. 1995 Nov;19(11):804-10.

Use of proton spectroscopy for detection of homozygous fatty ZDF-drt rats before weaning.

Author information

1
Department of Internal Medicine, Gifford Center for Diabetes Research, Dallas, Texas 75235, USA.

Abstract

OBJECTIVE:

To investigate the use of proton spectroscopy to measure abdominal fat content in pre-obese offspring of obese and lean Zucker diabetic fatty (ZDF-drt) rats.

DESIGN:

Large and small litters (size 4-15, 127 pups total) were serially measured during the suckling period to assess their abdominal fat index (AFI) and subsequently identified as to their genotype (fa/fa obese or Fa/? lean).

MEASUREMENTS:

Body weight, abdominal fat index (derived from percent fat by proton spectroscopy x body weight). When the abdominal fat index was plotted vs body weight, the data fell along two regression lines that distinguished pre-obese from lean pups. Percent fat by proton spectroscopy was validated against chemical measurement.

RESULTS:

Genotypes of pre-obese and lean pups were distinguishable from 13 days of age onwards. The procedure allowed prediction of genotype with 98% accuracy at 12-15 days and was 100% reliable at 16 days and beyond. The rate of fat accretion in the pups correlated directly with genotype, litter size and caloric density of the mother's food.

CONCLUSION:

Unlike currently available methods, the technique is rapid, simple, non-invasive and has the additional advantage of providing a measure of fat content sequentially over time in the same animal. It should be useful for the study of early metabolic derangements in the development of obesity and non-insulin dependent diabetes syndromes.

PMID:
8589782
[Indexed for MEDLINE]

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