Format

Send to

Choose Destination
Mol Plant Microbe Interact. 1996 Jan;9(1):74-7.

Isolation and characterization of a pSym locus of Rhizobium sp. BR816 that extends nodulation ability of narrow host range Phaseolus vulgaris symbionts to Leucaena leucocephala.

Author information

1
F. A. Janssens Laboratory of Genetics, KU Leuven, Heverlee, Belgium.

Abstract

Introduction of a cosmid library of megaplasmid DNA of Rhizobium sp. BR816, a broad host range Rhizobium strain, into R. etli CE3, a narrow host range bean symbiont, resulted in the isolation of a transconjugant that could effectively nodulate Leucaena leucocephala. Analysis of the corresponding cosmid, pBRF2, revealed the presence of genes required for elicting nitrogen-fixing nodules on L. leucoephala. Subcloning and Tn5 tagging identified a locus responsible for the host range extension. Sequence analysis of this locus revealed an ORF that shows significant identity with NodO of R. leguminosarum bv. viciae.

PMID:
8589426
DOI:
10.1094/mpmi-9-0074
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center