Effect of temperature on spontaneous Ca2+-activated Cl- currents in rabbit portal vein cells

Pflugers Arch. 1995 Nov;431(1):28-31. doi: 10.1007/BF00374374.

Abstract

The effect of temperature on the time course of spontaneous transient inward (Ca2+-activated Cl-) currents (STICs) was investigated with the perforated patch technique in rabbit portal vein cells. STICs decayed exponentially at temperatures between 17 degrees C and 35 degrees C. The time constant of the decay (tau ) was decreased with increasing temperature and had a temperature coefficient, Q10, of 2.6. The temperature sensitivity of tau could be described by the Arrhenius equation which gave an activation energy of 16kcal/mol. This result indicates that STIC decay is governed by a single first-order reaction and provides further support for the hypothesis that the decay is due to the gating properties of Ca2+-activated Cl- channels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / physiology*
  • Cell Membrane / physiology
  • Chloride Channels / metabolism*
  • Electrophysiology
  • In Vitro Techniques
  • Membrane Potentials / physiology
  • Muscle, Smooth, Vascular / metabolism*
  • Patch-Clamp Techniques
  • Portal Vein / metabolism*
  • Rabbits
  • Temperature

Substances

  • Chloride Channels
  • Calcium