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Insect Biochem Mol Biol. 1995 Dec;25(10):1061-5.

A new dominant selectable marker for genetic transformation; Hsp70-opd.

Author information

1
Centers for Disease Control and Prevention, Division of Parasitic Diseases, Atlanta, GA 30341, USA.

Abstract

New P element plasmids containing the organophosphate-degrading gene opd as a dominant selectable marker were tested as transformation vectors in Drosophila melanogaster. One of these vectors was modified by the addition of the D. melanogaster mini-white gene as a comarker. When transformed individuals were identified using paraoxon selection for opd alone, results were similar to those obtained with mini-white. No false positives were recovered, however one strain contained the mini-white gene but inadequate resistance to survive our screening regimen due to a defective Hsp70-opd gene. Results suggest that Hsp70-opd is similar to mini-white for distinguishing transformed individuals but does not require time-consuming individual examination. Due to the mode of action of organophosphorus nerve agents, Hsp70-opd has potential as a selectable marker in numerous animals beside fruit flies.

PMID:
8580907
DOI:
10.1016/0965-1748(95)00061-5
[Indexed for MEDLINE]

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