In this work, the seric and hypophysial concentrations of the hormones alpha-melantopin and prolactin of male specimens of Leptodactylus ocellatus from the province of Cordoba are studied during a complete annual period. This amphibian presents a continuous reproductive cycle with a wave of greater spermatogenic activity in early summer months (December-January). The results corresponding to hormones show a very similar pattern: both present their maximal seric value in the December-January period, coincidently with the greatest spermatogenic wave. Maximal hypophysial values precede seric concentration maximum by two months. Although the influence of these hormones on amphibian spermatogenesis is not well understood, the observations here described would indicate that alpha-melanotropin as well as prolactin could play a role in the spermatogenic process in Leptodactylus ocellatus.