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Chin Med Sci J. 1995 Sep;10(3):146-50.

The inhibitory effect of astragalus membranaceus on coxsackie B-3 virus RNA replication.

Author information

1
Shanghai Institute of Cardiovascular Diseases, Zhong Shan Hospital of Shanghai Medical University.

Abstract

Using mice infected with coxsackie B-3 virus (CVB3) as a viral myocarditis model, we observed the inhibitory effect of Astragalus membranaceus (AM) on CVB3-RNA replication in myocardial tissue of mice by RNA-RNA in situ hybridization with negative-strand RNA probes labelled with 35S and quantitative imaging analysis of positive signals. The mechanism of its effect on CVB3-RNA replication has been investigated by detection of beta-interferon (beta-IFN) as well. Results showed that the copy numbers of CVB3-RNA as well as the histologic scores (necrosis) in myocardial tissues of infected-AM treated mice were significantly lower than those in infected and normal saline treated mice, suggesting that AM could inhibit the replication of CVB3-RNA, but its effect on CVB3-RNA replication had no correlation with induction of beta-IFN.

PMID:
8580483
[Indexed for MEDLINE]

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