The prevalence of symptomatic mucocutaneous candidiasis in HIV-infected children is well documented. Information, however, of the carriage rate of potential fungal pathogens is lacking. In this study we determined the fungal colonization rate of multiple mucocutaneous sites from 13 HIV-infected and 12 control children. The rate of yeast and mould colonization and the species of fungal isolates were essentially the same for both groups of patients. However, several HIV-infected children asymptomatic for thrush proved to be colonized by Candida albicans, and disseminated colonization with Trichosporon beigelii occurred in one HIV-infected child. All cultures for dermatophytes were negative. While the carriage rate with fungi other than C. albicans was not increased in the HIV-infected group, the isolates recovered are known pathogens in the immunocompromised host and the colonization of these organisms may be a potential source of infection.