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Res Microbiol. 1995 Sep;146(7):587-94.

DNA fingerprinting by random amplified polymorphic DNA and restriction fragment length polymorphism is useful for yeast typing.

Author information

1
Dipartimento di Biologia Animale e Genetica Leo Pardi, Università degli Studi, Firenze, Italy.

Abstract

Random amplified polymorphic DNA (RAPD) analysis was applied to genomic DNA from nineteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces. Results obtained with five primers indicated that this technique is a powerful tool for yeast differentiation and identification. The data were consistent with those derived from restriction fragment length polymorphism (RFLP) using two S. cerevisiae DNA probes. We conclude that RAPD fingerprinting, combined with the analysis of RFLP, can provide unambiguous type assignment in yeasts.

PMID:
8578000
DOI:
10.1016/0923-2508(96)80565-1
[Indexed for MEDLINE]

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