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J Virol Methods. 1995 Sep;55(1):105-10.

Comparison of conventional autoradiography with a new DNA enzyme immunoassay for the detection of hepatitis C virus-polymerase chain reaction amplification products.

Author information

1
Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Eppendorf, Hamburg, Germany.

Abstract

The detection of HCV-PCR amplification products by DNA enzyme immunoassay (DEIA) was compared with conventional hybridization carried out with a 32P-labelled oligonucleotide probe. The detection limit of both methods was shown to be between 100 pg and 1 ng of amplicon. All serum samples of 40 HCV-seropositive patients were positive after PCR in autoradiography, but only 38 with the DEIA technique (sensitivity 95%). There were no false-positive reactions by either method. The advantage of the DEIA method was the fast and non-radioactive detection of HCV amplicons. DEIA combines the specificity of the hybridization event with the speed of an ELISA procedure and is suitable for HCV-PCR.

PMID:
8576299
DOI:
10.1016/0166-0934(95)00049-z
[Indexed for MEDLINE]

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