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Eur J Pharmacol. 1995 Jul 18;290(2):69-73.

Aldosterone inhibits nitric oxide synthesis in rat vascular smooth muscle cells induced by interleukin-1 beta.

Author information

1
Department of Cardiology, Jichi Medical School, Tochigi, Japan.

Abstract

We investigated the effects of aldosterone on nitric oxide (NO) synthesis in vascular smooth muscle cells. We measured the production of nitrite, a stable metabolite of NO, and the expression of inducible NO synthase mRNA and protein in cultured rat vascular smooth muscle cells. Incubation of the cultures with interleukin-1 beta (10 ng/ml) for 24 h caused a significant increase in nitrite generation. The interleukin-1 beta-induced nitrite production by vascular smooth muscle cells was significantly inhibited by aldosterone in a dose (10(-9) approximately 10(-6) M)-dependent manner. Incubation with interleukin-1 beta for 12 approximately 24 h caused inducible NO synthase mRNA expression in vascular smooth muscle cells, whereas aldosterone had a suppressive effect on its expression. Aldosterone also decreased interleukin-1 beta-induced NO synthase protein accumulation. These results indicate that aldosterone inhibits NO synthesis under interleukin-1 beta-stimulated conditions in vascular smooth muscle cells.

PMID:
8575535
DOI:
10.1016/0922-4106(95)90018-7
[Indexed for MEDLINE]

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